RETROVIRUS VECTORS DISPLAYING THE IGG-BINDING DOMAIN OF PROTEIN-A

We have designed and constructed retrovirus particles displaying the I gG-binding domain of protein A. We fused the gene for the synthetic an tibody-binding portion of protein A with the envelope gene of ecotropi c Moloney murine leukemia virus. The fusion gene was coexpressed in ec otropic retroviral packaging cells, and retrovirus particles with IgG- binding activities mere recovered. In principle, the protein A-envelop e chimeric retrovirus complexed with specific monoclonal antibody coul d be used for cell-targeted gene delivery. (C) 1997 Academic Press.