STARVATION-INDUCED GENES OF THE TOMATO PATHOGEN CLADOSPORIUM-FULVUM ARE ALSO INDUCED DURING GROWTH IN PLANTA

The pathogenicity of fungal pathogens is presumably dependent on genes that are expressed during infection, In order to isolate such genes f rom the tomato pathogen Cladosporium fulvum, and to test the hypothesi s that starvation-induced genes are also plant induced, a cDNA library was prepared from mycelia grown in a defined medium and then transfer red to a starvation medium. The library was then screened with cDNA pr obes prepared from starved and replete fungal mycelium, Five unique, d ifferentially expressed cDNAs were isolated from 1,000 clones screened , Northern (RNA) hybridization confirmed that all five were starvation induced, Interestingly, all five were also found to be plant induced, The identity of two of the clones was indicated by partial DNA sequen cing as alcohol and aldehyde dehydrogenase. The observed correlation b etween starvation induction and plant induction is discussed.