PURIFIED METALLOPROTEASE FROM THE PATHOGENIC HEMOFLAGELLATE CRYPTOBIA-SALMOSITICA AND ITS IN-VITRO PROTEOLYTIC ACTIVITIES

Authors
ZUO X WOO PTK
Citation
X. Zuo et Ptk. Woo, PURIFIED METALLOPROTEASE FROM THE PATHOGENIC HEMOFLAGELLATE CRYPTOBIA-SALMOSITICA AND ITS IN-VITRO PROTEOLYTIC ACTIVITIES, Diseases of aquatic organisms, 30(3), 1997, pp. 177-185
Citations number
35
Categorie Soggetti
Veterinary Sciences","Marine & Freshwater Biology
Journal title
Diseases of aquatic organisms → ACNP
ISSN journal
01775103
Volume
30
Issue
3
Year of publication
1997
Pages
177 - 185
Database
ISI
SICI code
0177-5103(1997)30:3<177:PMFTPH>2.0.ZU;2-H
Abstract
The metallo-protease (200 kDa) from Crytobia salmositica was purified using ion-exchange chromatography and gelfiltration. The purity of the enzyme was confirmed as there was only one homogeneous band using SDS -PAGE. Under in vitro conditions the purified metallo-protease complet ely degraded the extracellular matrix proteins (collagen types I, TV, V and laminin) and the membrane proteins isolated from rainbow trout e rythrocytes. The results confirm that the C. salmositica metallo-prote ase is a histolytic enzyme and it contributes to salmonid cryptobiosis and to the direct transmission of the parasite between fish.