DIETARY ENERGY RESTRICTION DOES NOT INHIBIT PANCREATIC CARCINOGENESISBY N-NITROSOBIS-2-(OXOPROPYL)AMINE IN THE SYRIAN-HAMSTER

Dietary energy restriction was previously shown to be effective in pre venting a wide range of experimentally induced cancers, Studies were c onducted to assess the influence on pancreatic carcinogenesis of dieta ry energy restrictions (reduced fat and carbohydrate) of 10%, 20% or 4 0% in comparison with control in Syrian hamsters treated with N-nitros obis(2-oxopropyl)amine (BOP), Two carcinogenesis studies were conducte d. One used a single treatment with 20 mg BOP/kg body weight and follo wed hamsters for 102 weeks following treatment, and the other used thr ee weekly treatments of 20 mg BOP/kg body weight and followed hamsters for 45 weeks after treatment, Hamsters were fed control or energy res tricted diet beginning the week following the last BOP treatment, Panc reatic carcinomas were induced in 9-18% of the hamsters in the first e xperiment and in 59-66% of the animals in the second, Dietary energy r estriction did not influence carcinoma incidence in either study, and in the second experiment the multiplicity of tumors was higher in the 40% energy restriction (ER) group than in control hamsters, Plasma cor ticosterone was suppressed by BOP treatment, particularly in the 20% a nd 40% ER hamsters in the second experiment, and diet or BOP treatment did not significantly alter plasma cortisol, Pancreatic protein kinas e c zeta measured by Western blot was highest in the cytosol and parti culate fractions of the 40% ER hamsters in the first experiment, These results indicate that dietary energy restriction is not effective in the prevention of BOP induced pancreatic carcinogenesis in the Syrian hamster.