M. Hosny et al., BASE COMPOSITION OF DNA FROM GLOMALEAN FUNGI - HIGH AMOUNTS OF METHYLATED CYTOSINE, Fungal genetics and biology, 22(2), 1997, pp. 103-111
Glomales (Zygomycetes) are obligate fungal symbionts of roots of land
plants and form arbuscular mycorrhiza, Sporal DNA of 10 isolates belon
ging to nine species was purified and the base composition was determi
ned by RP-HPLC, Base composition fell in a narrow range between 30 and
35% G + C. A high amount of methylated cytosine (mC) accounting for 2
-4% of the total nucleotides was found in all taxa, The DNA melting pr
ofile was defined for Scutellospora castanea, It corresponded to 32% G
i + C, and the shape of the denaturation curve suggested a heterogenei
ty in the GC content within the fungal genome, Knowledge of GC content
s and variations between taxa are essential for evaluating nuclear DNA
content using fluorimetric methods, and high proportions of mC/C + mC
in the genomes of glomalean fungi could reflect the existence of repe
ated DNA families, Results are discussed in relation to data for other
fungi and eukaryotes. (C) 1997 Academic Press.