CLONING AND NUCLEOTIDE-SEQUENCE OF A FULL-LENGTH CDNA-ENCODING RIBOSOMAL-PROTEIN L27 FROM HUMAN FETAL KIDNEY

Differential screening of a human fetal kidney cDNA library resulted i n the isolation of D69, eventualy renamed HumRPL27, which was expresse d at higher levels in fetal kidney than in adult kidney. The 476 bp cD NA insert from HumRPL27 contains an open reading frame of 135 amino ac ids displaying 100% identity to rat RPL27 and chicken RPL27 predicted protein sequences although 64 and 38 silent base pairs changes respect ively are found at the DNA level. In Northern blots, a 1.0 kb HumRPL27 mRNA transcript is expressed abundantly in all fetal tissues examined and at lower abundance in adult tissues. Southern analysis of HumRPL2 7 suggests the presence of multiple copies of the gene in human, rat, mouse and hamster DNA.