ISOLATION OF PROMOTER FOR CYTOSOLIC PHOSPHOLIPASE A(2) (CPLA2)

Cytosolic phospholipase A(2) (cPLA2) releases arachidonic acid from me mbrane phospholipids and is believed to be the rate-limiting enzyme in the arachidonic acid pathway. We report herein the isolation of a 3 k b fragment of rodent genomic DNA containing part of the first intron, the first exon and 5'-flanking sequence. The start site of transcripti on was mapped by 5'-rapid amplification of cDNA ends and corroborated by ribonuclease protection assay. The gene has a TATAless promoter wit h no classical Spl binding sites or initiator element. A microsatellit e series of CA repeats was noted in the 5'-flanking region of both the rodent and human promoters. Deletion constructs have been analysed fo r luciferase activity and confirmed promoter activity.