DETERMINATION OF BINDING AFFINITIES OF GLUCOSE-OXIDASE FOR SODIUM N-DODECYL SULFATE

Authors
HOUSAINDOKHT MR MOOSAVIMOVAHEDI AA
Citation
Mr. Housaindokht et Aa. Moosavimovahedi, DETERMINATION OF BINDING AFFINITIES OF GLUCOSE-OXIDASE FOR SODIUM N-DODECYL SULFATE, International journal of biological macromolecules, 16(2), 1994, pp. 77-80
Citations number
14
Categorie Soggetti
Biology
Journal title
International journal of biological macromolecules → ACNP
ISSN journal
01418130
Volume
16
Issue
2
Year of publication
1994
Pages
77 - 80
Database
ISI
SICI code
0141-8130(1994)16:2<77:DOBAOG>2.0.ZU;2-0
Abstract
The binding of sodium n-dodecyl sulfate (SDS) to glucose oxidase was s tudied at pH 3.2 and 6.4 by equilibrium dialysis at 37 degrees C. The binding data have been analysed in terms of Scatchard plots which show unusual minima. These plots were analysed for two interacting sets of sites. Equations for the free energy of binding of each set of sites were obtained by a combination of the Tanford and Hill theories to obt ain the affinities of each set of sites.