Mr. Housaindokht et Aa. Moosavimovahedi, DETERMINATION OF BINDING AFFINITIES OF GLUCOSE-OXIDASE FOR SODIUM N-DODECYL SULFATE, International journal of biological macromolecules, 16(2), 1994, pp. 77-80
The binding of sodium n-dodecyl sulfate (SDS) to glucose oxidase was s
tudied at pH 3.2 and 6.4 by equilibrium dialysis at 37 degrees C. The
binding data have been analysed in terms of Scatchard plots which show
unusual minima. These plots were analysed for two interacting sets of
sites. Equations for the free energy of binding of each set of sites
were obtained by a combination of the Tanford and Hill theories to obt
ain the affinities of each set of sites.