CHARACTERIZATION OF BOVINE PEROXISOME PROLIFERATOR-ACTIVATED RECEPTORS GAMMA-1 AND GAMMA-2 - GENETIC-MAPPING AND DIFFERENTIAL EXPRESSION OFTHE 2 ISOFORMS

In this report we describe the isolation and characterisation of the c DNAs encoding two isoforms of peroxisome proliferator-activated recept or gamma (PPAR gamma), gamma 1 and gamma 2, in cattle. The cDNA sequen ces show strong conservation with the corresponding sequences reported in other species. The distribution of PPAR gamma mRNAs in various bov ine tissues was investigated using Northern blot analysis. The highest expression was detected in adipose tissue with about equal amounts of the both transcripts while a differential expression was found in oth er tissues investigated. PPAR gamma 1 was expressed at relatively high levels in bovine spleen and lung and to a lower extent in ovary, mamm ary gland, and small intestine. The amount of PPAR gamma 2 was apparen tly lower than that of PPAR gamma 1 in spleen, lung, and ovary. These results indicate a modulation of tissue distribution for the two trans cripts in cattle. Using genetic linkage analysis we have also assigned the PPAR gamma gene to bovine chromosome 22. (C) 1997 Academic Press.