IDENTIFICATION OF 3 RANDOMLY AMPLIFIED POLYMORPHIC DNA-POLYMERASE CHAIN-REACTION MARKERS FOR DISTINGUISHING ASIAN AND NORTH-AMERICAN GYPSY MOTHS (LEPIDOPTERA, LYMANTRIIDAE)
De. Schreiber et al., IDENTIFICATION OF 3 RANDOMLY AMPLIFIED POLYMORPHIC DNA-POLYMERASE CHAIN-REACTION MARKERS FOR DISTINGUISHING ASIAN AND NORTH-AMERICAN GYPSY MOTHS (LEPIDOPTERA, LYMANTRIIDAE), Annals of the Entomological Society of America, 90(5), 1997, pp. 667-674
Gypsy moths originating in Asia have recently been introduced into Nor
th America, making it necessary to develop markers for distinguishing
the Asian strain from the established North American population. We ha
ve identified 3 randomly amplified polymorphic DNA-polymerase chain re
action generated (RAPD-PCR) markers which are specific for either Asia
n or North American moths. The FS-2 and FS-3 markers are specific for
the North American strain, and the FS-4 marker is specific for moths f
rom Ear east Asia. The 3 RAPD-amplified fragments have been cloned and
sequenced, and locus-specific primers have been developed for the FS-
3 marker. These markers, in combination with other markers already ava
ilable, will be useful for increasing the accuracy of identification o
f suspect moth specimens.