The reproducibility of AMPLICOR enterovirus PCR test results was deter
mined with clinical samples of cerebrospinal fluid, serum, urine, and
throat and rectal swabs. Among 608 samples from which duplicate aliquo
ts were run simultaneously, only seven pairs gave discordant results,
Among 104 samples from which duplicate aliquots were run in separate a
ssays, no discordance was seen. Overall, the reproducibility of test k
it results was 99% (705 of 712).