RAPID DISCRIMINATION OF MYCOBACTERIUM-TUBERCULOSIS COMPLEX STRAINS BYLIGATION-MEDIATED PCR FINGERPRINT ANALYSIS

Authors
PRODHOM G GUILHOT C GUTIERREZ MC VARNEROT A GICQUEL B VINCENT V
Citation
G. Prodhom et al., RAPID DISCRIMINATION OF MYCOBACTERIUM-TUBERCULOSIS COMPLEX STRAINS BYLIGATION-MEDIATED PCR FINGERPRINT ANALYSIS, Journal of clinical microbiology, 35(12), 1997, pp. 3331-3334
Citations number
17
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
35
Issue
12
Year of publication
1997
Pages
3331 - 3334
Database
ISI
SICI code
0095-1137(1997)35:12<3331:RDOMCS>2.0.ZU;2-#
Abstract
A ligation-mediated PCR (LMPCR) method for the amplification of sequen ces flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a s econd specific for a linker ligated to SalI-restricted genomic DNA. LM PCR is a rapid screening method, valuable for the fingerprinting of M. tuberculosis complex strains.