QUANTITATIVE MEASUREMENT OF PROTEIN-KINASE-C IMMUNOREACTIVITY IN ROD BIPOLAR CELLS OF THE GOLDFISH RETINA

Authors
VAQUERO CF VELASCO A DELAVILLA P
Citation
Cf. Vaquero et al., QUANTITATIVE MEASUREMENT OF PROTEIN-KINASE-C IMMUNOREACTIVITY IN ROD BIPOLAR CELLS OF THE GOLDFISH RETINA, Brain research, 773(1-2), 1997, pp. 208-212
Citations number
10
Categorie Soggetti
Neurosciences
Journal title
Brain research → ACNP
ISSN journal
00068993
Volume
773
Issue
1-2
Year of publication
1997
Pages
208 - 212
Database
ISI
SICI code
0006-8993(1997)773:1-2<208:QMOPII>2.0.ZU;2-S
Abstract
The intensity of the immunohistochemical reaction (IIR) against the cr species of protein kinase C (PKC) was quantified in the rod bipolar c ells (RBC) of the goldfish retina using of image analysis. Retinae inc ubated in control Ringer solution showed similar IIR in bath the soma and the axon terminal (IIR-ratio approximate to 1). Activation of PKC induces the 'transport' of the enzyme to the synaptic terminal of RBC and an increase in the IIR-ratio. In the present report, the effect of retinal neurotransmitters on the IIR-ratio and the time course of PKC transport was studied. (C) 1997 Elsevier Science B.V.