ALLIUM CHEMISTRY - SUPERCRITICAL-FLUID EXTRACTION AND LC-APCI-MS OF THIOSULFINATES AND RELATED-COMPOUNDS FROM HOMOGENATES OF GARLIC, ONION,AND RAMP - IDENTIFICATION IN GARLIC AND RAMP AND SYNTHESIS OF 1-PROPANESULFINOTHIOIC ACID S-ALLYL ESTER

Supercritical fluid (SF) extracts of homogenized garlic (Allium sativu m L.), ramp (A. tricoccum Ait.), chemical ionization mass spectrometri c identification. The major thiosulfinates from garlic and ramp were r eadily characterized by this technique. Small quantities of ajoene, a potent antithrombotic agent, were also found in SP extracts of garlic homogenates. The profiles of onion juice extracts revealed the usual t hiosulfinates, zwiebelanes, and bissulfine reported in prior studies, as well as cepaenes previously identified in extracts of onion juice t hrough extensive isolation steps and spectroscopic methods. The presen ce of trace quantities of allyl compounds in onion juice and propyl co mpounds in garlic and ramp-homogenates has been verified by LC-mass sp ectrometry (MS). The presence of these compounds was not readily evide nt in previous analyses using gas chromatography-MS with cold-on-colum n injection and reversed-phase or normal phase LC with UV detection.