INVOLVEMENT OF CYTOKINES IN THE HISTOPATHOLOGY OF CEREBRAL MALARIA

Histopathologic and immunohistologic studies were performed in two cas es of fatal cerebral malaria. On admission, both patients were in unar ousable coma with hyperparasitemia. Examination of the tissue sections from various organs showed parasite sequestration in both cases with more extensive area of sequestration in case I than in case 2. A panel of monoclonal antibodies against cytokines applied to these tissues c learly detected tumor necrosis factor-alpha (TNF alpha), interferon-ga mma (IFN gamma), interleukin-1 beta (IL-1 beta), and IL-10 in the tiss ues from brain and liver of case 1. A different cytokine profile, IL-4 and IL-10, was found in the brain tissues of case 2; no TNF alpha nor IFN gamma was detected. There was no cytokine detected in the tissues of other organs in either case. Results of the study suggest that his topathology in the brain of fatal cerebral malaria may be associated w ith focal accumulation of cytokines. Additionally, the type of cytokin es produced locally in a particular tissue during malaria infection ma y be regulated by the degree of regional parasite sequestration.