E. Strobel et T. Graf, DEPRESSION OF THE ANTIGEN-A AND ANTIGEN-H IN A PATIENT WITH ACUTE-LEUKEMIA, Infusionstherapie und Transfusionsmedizin, 24(5), 1997, pp. 354-358
Background: In the case of a 39-year-old woman, who suffered from acut
e myeloic leukemia and was treated for the first time, the examination
of the ABO blood group showed a discrepancy between the ABO antigens
on her red blood cells (RBCs) (no reaction with anti-A, anti-B and ant
i-AB reagents) and the isoagglutinins in her serum (no reaction with A
l, Az and O RBCs, but strong reaction with B RBCs). Materials and Meth
ods: 5 monoclonal and 5 polyclonal anti-A, anti-B and anti-AB reagents
of different manufacturers were used for the determination of the pat
ient's ABO blood group (tube-spin method,5 min incubation at room temp
erature). Results: No polyclonal and only 1 monoclonal anti-A, 3 polyc
lonal and 2 monoclonal anti-AB reagents were able to detect the A anti
gen. Furthermore the H antigen was not detectable with monoclonal and
with lectin reagents. Seven months later (after chemotherapy in the me
antime) the A and H antigens were found on her RBCs with strong reacti
ons. Conclusion: Even if there are excellent monoclonal and polyclonal
reagents for the examination of the ABO antigens on the RBCs, for the
determination of the ABO blood group the complementary reactions of t
he isoagglutinins must be carefully observed (except for newborns and
children younger than 1 year).