SUBCRITICAL-FLUID AND SUPERCRITICAL-FLUID EXTRACTION OF TRICHLOROPYRIDINOL FROM SOIL PRIOR TO IMMUNOASSAY

Citation
Mm. Jimenezcarmona et al., SUBCRITICAL-FLUID AND SUPERCRITICAL-FLUID EXTRACTION OF TRICHLOROPYRIDINOL FROM SOIL PRIOR TO IMMUNOASSAY, Journal of chromatography, 785(1-2), 1997, pp. 329-336
Citations number
20
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Volume
785
Issue
1-2
Year of publication
1997
Pages
329 - 336
Database
ISI
SICI code
Abstract
A comparative study on the extraction of TCP (3,5,6-trichloro-2-pyridi nol, a metabolite of chlorpyrifos) from soil with CO2 and H2O is repor ted. The polarity of the analyte requires the presence of both a cosol vent (methanol) and an ion-pair reagent [(1R)-(-)-10-camphorsulfonic a cid ammonium salt] for 95% extraction in 30 min when supercritical CO2 at 40 degrees C and 383 bar is used as extractant. Subcritical water (250 degrees C and 200 bar) enables complete extraction within 15 min without additives. Quantitation of the target analyte is performed by specific immunoassay using a non-commercial monoclonal antibody which provides a linear determination range between 0.005 and 5 mu g/g, with coefficients of variation of 5.3 and 4.9% for the SC-CO2 and sub-H2O extractions, respectively. (C) 1997 Elsevier Science B.V.