Mm. Jimenezcarmona et al., SUBCRITICAL-FLUID AND SUPERCRITICAL-FLUID EXTRACTION OF TRICHLOROPYRIDINOL FROM SOIL PRIOR TO IMMUNOASSAY, Journal of chromatography, 785(1-2), 1997, pp. 329-336
Citations number
20
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A comparative study on the extraction of TCP (3,5,6-trichloro-2-pyridi
nol, a metabolite of chlorpyrifos) from soil with CO2 and H2O is repor
ted. The polarity of the analyte requires the presence of both a cosol
vent (methanol) and an ion-pair reagent [(1R)-(-)-10-camphorsulfonic a
cid ammonium salt] for 95% extraction in 30 min when supercritical CO2
at 40 degrees C and 383 bar is used as extractant. Subcritical water
(250 degrees C and 200 bar) enables complete extraction within 15 min
without additives. Quantitation of the target analyte is performed by
specific immunoassay using a non-commercial monoclonal antibody which
provides a linear determination range between 0.005 and 5 mu g/g, with
coefficients of variation of 5.3 and 4.9% for the SC-CO2 and sub-H2O
extractions, respectively. (C) 1997 Elsevier Science B.V.