2 DISTINCT ANTIALLERGIC DRUGS, AMLEXANOX AND CROMOLYN, BIND TO THE SAME KINDS OF CALCIUM-BINDING PROTEINS, EXCEPT CALMODULIN, IN BOVINE LUNG EXTRACT

In order to explore candidates for proteins required in exocytosis, we used two anti-allergic drugs, amlexanox and cromolyn, which inhibit I gE mediated degranulation of mast cells and basophils, as molecular pr obes in affinity chromatography. These two drugs chiefly bound to the same kinds of calcium binding proteins in bovine lung, These proteins were as follows: bovine calgranulin C homolog, an 8-kDa unknown protei n, S-100L, calgranulin B, calcyphosine, and annexins I-V, The homologo us affinity of the two drugs to these proteins is in accord with the s imilar anti-allergic property of both drugs. From these findings it is presumed that these drugs interact with these proteins and affect pha rmacologically the degranulation. (C) 1997 Academic Press.