CLONING OF P2Y6 CDNAS AND IDENTIFICATION OF A PSEUDOGENE - COMPARISONOF P2Y RECEPTOR SUBTYPE EXPRESSION IN BONE AND BRAIN-TISSUES (VOL 237, PG 297, 1997)

Cellular responses to ATP/UTP and analogs are mediated by G-protein co upled P2Y receptors and have been proposed to play a role in the regul ation of bone metabolism. Using a degenerate PCR approach on MG-63 cel l cDNA me found PCR fragments coding for human P2Y1 and a new receptor , P2Y6. cDNA cloning of the P2Y6 receptor identified three cDNA isofor ms. Two contained the same contiguous ORFs but differed in their 5' UT Rs and may therefore originate by alternative splicing whereas the thi rd represents a pseudogene. Analysis of P2Y receptor subtype expressio n in human bone and the osteoblastic cell lines OHS-4 and MG-63 by RT- PCR showed that all known human P2Y receptor subtypes (P2Y1, P2Y2, P2Y 4, P2Y6, and P2Y7) were expressed. In contrast, analysis of brain-deri ved cell. Lines suggests that a selective expression of P2Y receptor s ubtypes occurs in brain tissue. (C) 1997 Academic Press.