Dentinal tubules of human root canal walls were infected with a known
bacterial isolate, The teeth were divided into two groups and the root
canals instrumentated, Different types of canal irrigant were used fo
r each group, In group A, 5% NaOCl was followed by a 10% EDTA rinse an
d neutralized with a final physiological solution rinse, In Group B, 1
0% EDTA, a tensioactive agent (TRITON), and 5% NaOCl were used in sequ
ence, with a final physiological solution rinse to neutralize the acti
on of the agents used, Histological examination of group A specimens s
howed a residual area of infection extending from the canal lumen to a
mean depth of 300 mu m. Histological examination of group B specimens
showed an infection-free area of tubules to a mean depth of 130 mu m.
Below this was an infected area of variable extent, In some group B s
ections, no infection was found.