REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION ANDQUANTIFICATION OF LANTADENES USING ISOCRATIC SYSTEMS

Isocratic systems of varying suitability are reported for reversed-pha se HPLC of lantadenes, the pentacyclic triterpenoids from the hepatoto xic plant Lantana camara var. aculeata. The column used was a Nova-Pak C-18,, (250x4.6 mm). The detection was at 210 nm and 240 nm. The mobi le phase acetonitrile-water-acetic acid (80:20:0.01) provided baseline separation of all the lantadenes except lantadenes A and C. The mobil e phase methanol-water-acetic acid (85:15:0.01) also provided resoluti on of ail the lantadenes but the retention time windows were very broa d. The mobile phase methanol-acetonitrile-water-acetic acid (68:20:12: 0.01) has been found to be the most suitable for the separation and qu antitation of lantadenes. (C) 1997 Elsevier Science B.V.