DETERMINATION OF MAGNOLOL AND HONOKIOL IN MAGNOLIAE CORTEX USING SUPERCRITICAL-FLUID CHROMATOGRAPHY ONLINE COUPLED WITH SUPERCRITICAL-FLUIDEXTRACTION BY ON-COLUMN TRAPPING

An assay of magnolol and honokiol in Magnoliae Cortex was established by on-line supercritical fluid chromatography (SFC) coupled with super critical fluid extraction (SFE). With an amino column as the trapping and separation column, magnolol and honokiol were extracted and focuse d at the column head as a narrow band, even if 5% methanol was added t o the supercritical carbon dioxide as an entrainer. Addition of the en trainer improved extract efficiency, and recovery of the analytes was almost the same as for solvent extraction, Determination of analytes o f interest in Magnoliae Cortex was achieved with only a few mg of samp le. SFE was completed within ca. 1 min, and SFC was achieved within ca . 3 min. The consecutive procedures of extraction, concentration and a nalysis were completed within ca. 5 min. (C) 1997 Elsevier Science B.V .