PROTEIN-A CARRYING PMMA MICROBEADS - ADSORPTION OF CHOLESTEROL AND HIGG FROM HUMAN PLASMA

Cholesterol and HlgG adsorbed from human plasma obtained from a hyperc holesterolemic patient, onto protein A-immobilized polymethyl-methacry late uniform microbeads carrying different amounts of protein A (0.264 -1.682 mg protein A/g PMMA, or 0.66-4.2 mg protein A/m(2) PMMA) were i nvestigated in batchwise experiments. There was no interaction between protein A molecules and cholesterol when cholesterol aqueous solution s were used. However, there was significant cholesterol and HlgG adsor ption from the plasma obtained from a patient with hypercholesterolemi a. The maximum amounts of cholesterol and HlgG adsorbed were 3.96 mu m ol cholesterol/g PMMA (5.4 mg cholesterol/g PMMA) and 0.242 mu mol IgG /g PMMA (35.4 mg IgG/g PMMA).