HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR IBUPROFEN IN WHOLE-BLOOD USING SOLID-PHASE EXTRACTION

Authors
SOCHOR J KLIMES J ZAHRADNICEK M SEDLACEK J
Citation
J. Sochor et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR IBUPROFEN IN WHOLE-BLOOD USING SOLID-PHASE EXTRACTION, Journal of chromatography B. Biomedical applications, 654(2), 1994, pp. 282-286
Citations number
28
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
654
Issue
2
Year of publication
1994
Pages
282 - 286
Database
ISI
SICI code
Abstract
A precise, accurate, reproducible one-step method for the high-perform ance chromatographic determination of ibuprofen in whole blood is desc ribed. Samples were, after haemolysis, prepared by solid-phase extract ion. Analyses were performed using reversed-phase chromatography on a Separon SGX C-18 column with a mobile phase of methanol-water (pH 3) a nd ultraviolet detection at 222 nm. The method was used for pharmacoki netic studies in rabbits.