ANTIIDIOTYPIC MONOCLONAL-ANTIBODIES AGAINST ANTIOVARIAN CARCINOMA MONOCLONAL-ANTIBODY COC166-9 - GENERATION AND APPLICATION

Anti-idiotypic monoclonal antibody (Mab Ab2) by MAb COC166-9 against o varian serous papillary adenocarcinoma was prepared. Hybridomas of Ab2 screened by sandwich ELISA and immunocompetitive inhibition tests wer e procured and named as 6B11 and 1H12. The number of their chromosomes were 93 and 91, and DNA analysis also proved the characteristics of h ybridomas. These Ab2s could induce delayed type hypersensitivity (DTH) , the cellular immune response. The results of the immune reaction of 6B11 with SKOV3 (ovarian carcinoma cell line) were similar to OC166-9 (Ag), the positive control, while 1H12 was weaker. Anti-anti-idiotypic antibody (Ab3) was also raised by 6B11 and 1H12 respectively. They al l showed positive immunohistochemical stainings with ovarian serous ad enocarcinoma tissue sections and immunocytochemical stainings with SKO V3 cells as was shown by COC166-9. In the antibody dependent cell medi ated cytotoxicity (ADCC) tests, they showed no differences against SKO V3 as compared with COC166-9. We anticipate that 6B11 and 1H12 may be used as vaccines against ovarian carcinoma and may provide a clue for its prevention and treatment.