A COMPARATIVE-ANALYSIS BETWEEN [P-32] AND DIGOXIGENIN-LABELED SINGLE-COPY PROBES FOR RFLP DETECTION IN WHEAT

The RFLP technique is widely used in plant-genome mapping and other ge netic analyses. While it has great potential as a tool for plant breed ers, the requirement for radioisotopes has limited its routine use. He re, we have used a commercially available nonisotopic method, the digo xigenin-labelling and detection system (Boehringer Mannheim), with mod ifications, to analyse a complex polyploid genome such as wheat, and h ave obtained results equivalent to the isotopic method. The detailed e xperimental procedures are described. This nonisotopic method will fac ilitate various RFLP-based genome analyses.