M. Gjomarkaj et al., DENDRITIC CELLS WITH A POTENT ACCESSORY ACTIVITY ARE PRESENT IN HUMANEXUDATIVE MALIGNANT PLEURAL EFFUSIONS, The European respiratory journal, 10(3), 1997, pp. 592-597
Dendritic cells are human leucocyte antigen (HLA)-DR positive accessor
y cells, that play a critical role in the development of cell-mediated
immune reactions. Since the pleural space is frequently involved in c
ell-mediated immune reactions, we sought to isolate dendritic cells fr
om pleural fluid. Pleural effusion mononuclear cells (PEMCs) were obta
ined by Ficoll centrifugation of exudative pleural effusions recovered
from 19 patients with malignant pleurisy. After double-step adherence
, firmly-adherent mononuclear cells (FAMs) and loosely-adherent mononu
clear cells (LAMs) were recovered. The latter cells were centrifuged o
n a bovine plasma albumin gradient to obtain a loosely-adherent low de
nsity fraction. Nonadherent cells were rosetted with sheep red blood c
ells (SRBC) to obtain a nonadherent nonrosetting (NANR) cell fraction.
Mitomycin C-treated PEMCs, NANRs, FAMs, and LAMs served as stimulator
y cells in mixed leucocyte reaction experiments. H-3-thymidine incorpo
ration by purified normal allogeneic blood T-lymphocytes was assessed
as an index of accessory cell function. The phenotype of NANR, FAM and
LAM cells was characterized using single and double stainings with a
panel of monoclonal antibodies (MoAbs). Accessory cell (AC) activity (
counts per minute (cpm) x10(-3)(+/-SE); 2.5 x 10(4) AC . well(-1)) of
LAM (148+/-24) and NANR (108+/-11.2) was greater than that observed fo
r FAM (59.3+/-9.4) and for unfractioned PEMC (13.8+/-4.9). The FAM fra
ction was virtually entirely composed of CD68+ HLA-DR+ mononuclear pha
gocytes. NANR and LAM contained 51+/-12% and 65+/-6% HLA-DR+ cells, re
spectively, and most HLA-DR positive cells were negative for CD3, CD19
, and CD68, markers for T-, B-lymphocytes and mononuclear phagocytes.
Moreover, both NANR and LAM fractions contained significant numbers of
cells bearing the RFD1 surface marker, expressed on dendritic cells.
These results suggest that dendritic cells are present in exudative pl
eural effusions, and that they may be involved in the development of c
ell-mediated immune reactions in the pleural space.