We used genetic analysis to examine the mating strategies of male and
female Eastern Screech-Owls (Otus asio) in central Kentucky. DNA finge
rprinting revealed no evidence of extra-pair fertilizations in 23 broo
ds (80 nestlings). Such results suggest that pursuit of extra-pair cop
ulations by male and female screech-owls may be costly. One possible c
ost for females is the risk of losing the nest site. Alternatively, pu
rsuit of extra-pair matings by females might be energetically expensiv
e, thereby conflicting with egg production. Male screech-owls provide
food for their mate (and young) during much of the breeding season and
such feeding probably affects reproductive success. Males pursuing EP
Cs might have less time for foraging and, as a result, reduced reprodu
ctive success.