A DOMAIN-CONTAINING THE CDC42 RAC INTERACTIVE BINDING (CRIB) REGION OF P65(PAK) INHIBITS TRANSCRIPTIONAL ACTIVATION AND CELL-TRANSFORMATIONMEDIATED BY THE RAS-RAC PATHWAY/

Authors
OSADA S IZAWA M KOYAMA T HIRAI S OHNO S
Citation
S. Osada et al., A DOMAIN-CONTAINING THE CDC42 RAC INTERACTIVE BINDING (CRIB) REGION OF P65(PAK) INHIBITS TRANSCRIPTIONAL ACTIVATION AND CELL-TRANSFORMATIONMEDIATED BY THE RAS-RAC PATHWAY/, FEBS letters, 404(2-3), 1997, pp. 227-233
Citations number
42
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
404
Issue
2-3
Year of publication
1997
Pages
227 - 233
Database
ISI
SICI code
0014-5793(1997)404:2-3<227:ADTCRI>2.0.ZU;2-9
Abstract
The molecular bases of the versatile functions of Rho-like GTPases are still unknown. Using luciferase assays with rat 3Y1 cells, we found t hat Rad is integrated downstream of Ras in the TRE (TPA response eleme nt) activation pathway. Coexpression of a mutant of p65(PAK), PAK/RD, lacking the kinase domain but containing the Cdc42/Rac interactive bin ding (CRIB) region, suppressed the TRE activation and cell transformat ion caused by constitutively activated forms of Ras (RasV12) and Rac1 (Rac1V12). PAK/RD is a good tool to investigate the signaling pathways in which Rac and Cdc42 are involved. (C) 1997 Federation of European Biochemical Societies.