EFFECT OF ANTIFREEZE PROTEINS ON FROZEN PRIMARY PROSTATIC ADENOCARCINOMA CELLS

Citation
H. Koushafar et B. Rubinsky, EFFECT OF ANTIFREEZE PROTEINS ON FROZEN PRIMARY PROSTATIC ADENOCARCINOMA CELLS, Urology, 49(3), 1997, pp. 421-425
Citations number
16
Categorie Soggetti
Urology & Nephrology
Journal title
ISSN journal
00904295
Volume
49
Issue
3
Year of publication
1997
Pages
421 - 425
Database
ISI
SICI code
0090-4295(1997)49:3<421:EOAPOF>2.0.ZU;2-A
Abstract
Objectives. Recent studies show that prostate adenocarcinoma cells can survive cryosurgery and that cell destruction depends on the specific thermal parameters used during freezing. The goal of this preliminary study is to determine whether certain chemical compounds, known as an tifreeze proteins, can induce com-; plete human primary prostatic aden ocarcinoma cell destruction by freezing, regardless of the thermal par ameters used. The study also examines the mechanism by which antifreez e proteins bring about cell destruction. Methods. Antifreeze proteins were added to solutions containing human primary prostatic adenocarcin oma cells. The cells were frozen with controlled thermal parameters us ing a directional solidification apparatus attached to a light microsc ope. Cell viability was determined after thawing as a function of anti freeze protein concentration and cooling rate during freezing. Results . The dose response study shows that for all the cooling rates tested, 10-mg/mL solutions of antifreeze protein cause the complete destructi on of human primary prostatic adenocarcinoma cells frozen to a tempera ture at which, without these proteins, the cells survive freezing. Lig ht microscopy shows that the lethal effect of the antifreeze proteins is related to the formation of intracellular ice in the frozen cells. Conclusions. This preliminary study has demonstrated that antifreeze p roteins have the ability to generate complete destruction of prostatic adenocarcinoma cells frozen to high subzero temperatures irrespective of the cooling rates used during freezing. This suggests that introdu cing antifreeze proteins into undesirable tissues prior to freezing ma y increase the efficacy and the control over tissue destruction by cry osurgery. (C) 1997, Elsevier Science Inc.