A FLUOROMETRIC-DETERMINATION METHOD FOR D,L CONFIGURATIONS OF PER-O-METHYLATED MONOSACCHARIDES BY ANOMERIC 2-METHYL-2-BETA-NAPHTHYL-1,3-BENZODIOXOLE 4-CARBOXYLATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
BAI C OHRUI H NISHIDA Y MEGURO H
Citation
C. Bai et al., A FLUOROMETRIC-DETERMINATION METHOD FOR D,L CONFIGURATIONS OF PER-O-METHYLATED MONOSACCHARIDES BY ANOMERIC 2-METHYL-2-BETA-NAPHTHYL-1,3-BENZODIOXOLE 4-CARBOXYLATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Analytical biochemistry, 246(2), 1997, pp. 246-252
Citations number
15
Categorie Soggetti
Biology
Journal title
Analytical biochemistry → ACNP
ISSN journal
00032697
Volume
246
Issue
2
Year of publication
1997
Pages
246 - 252
Database
ISI
SICI code
0003-2697(1997)246:2<246:AFMFDC>2.0.ZU;2-E
Abstract
Per-O-methylated pentopyranoses and hexopyranoses were converted to th eir glycosyl chlorides and coupled with cesium salt of a fluorescent c hiral derivatization reagent, thyl-2-beta-naphthyl-1,3-benzodioxole-4- carboxylic acid [(+)-MNB carboxylic acid], to afford their 1-O-(s)-MNB carboxylates. The D,L enantiomers were separated by normal-phase HPLC and determined at. the picomolar level, and this methodology could be extended to the highly sensitive simultaneous determination of per-O- methylated monosaccharides. (C) 1997 Academic Press.