A FLUOROMETRIC-DETERMINATION METHOD FOR D,L CONFIGURATIONS OF PER-O-METHYLATED MONOSACCHARIDES BY ANOMERIC 2-METHYL-2-BETA-NAPHTHYL-1,3-BENZODIOXOLE 4-CARBOXYLATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
C. Bai et al., A FLUOROMETRIC-DETERMINATION METHOD FOR D,L CONFIGURATIONS OF PER-O-METHYLATED MONOSACCHARIDES BY ANOMERIC 2-METHYL-2-BETA-NAPHTHYL-1,3-BENZODIOXOLE 4-CARBOXYLATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Analytical biochemistry, 246(2), 1997, pp. 246-252
Per-O-methylated pentopyranoses and hexopyranoses were converted to th
eir glycosyl chlorides and coupled with cesium salt of a fluorescent c
hiral derivatization reagent, thyl-2-beta-naphthyl-1,3-benzodioxole-4-
carboxylic acid [(+)-MNB carboxylic acid], to afford their 1-O-(s)-MNB
carboxylates. The D,L enantiomers were separated by normal-phase HPLC
and determined at. the picomolar level, and this methodology could be
extended to the highly sensitive simultaneous determination of per-O-
methylated monosaccharides. (C) 1997 Academic Press.