EFFECT OF CALCIUM-IONS ON THE IRRADIATION-INDUCED INACTIVATION OF CELLULASE

Authors
KUMAKURA M
Citation
M. Kumakura, EFFECT OF CALCIUM-IONS ON THE IRRADIATION-INDUCED INACTIVATION OF CELLULASE, Isotopes in environmental and health studies, 32(4), 1996, pp. 411-419
Citations number
14
Categorie Soggetti
Chemistry Inorganic & Nuclear","Environmental Sciences
Journal title
Isotopes in environmental and health studies → ACNP
ISSN journal
10256016
Volume
32
Issue
4
Year of publication
1996
Pages
411 - 419
Database
ISI
SICI code
1025-6016(1996)32:4<411:EOCOTI>2.0.ZU;2-M
Abstract
The activity of cellulase irradiated at various temperatures was exami ned as a function of irradiation dose. The effect of calcium ions in r adiation inactivation of cellulase at irradiation temperature of 30 de grees C was studied by using calcium sulfate. Tile calcium ions have a protective ability against radiation caused inactivation of cellulase by scavenging species such as OH- formed by irradiation of cellulase aqueous solution. in which the effective concentration range of the ca lcium ions was similar to-10(-3) M. The calcium ions do not act For th e heat inactivation of the enzyme and the enzyme hydrolysis of filter paper or chaff as an activator because the calcium ions do not associa te with the enzyme to form a calcium ion-enzyme complex.