VOLTAMMETRIC CHARACTERISTICS OF ADENINE AND ADENOSINE AT A COPPER ELECTRODE AND THEIR APPLICATION TO END-COLUMN AMPEROMETRIC DETECTION FOR CAPILLARY ZONE ELECTROPHORESIS

The voltammetric characteristics of adenine and adenosine at a copper electrode were investigated in different supporting electrolytes. It w as found that citric acid had some activating effect at the copper ele ctrode and the oxidation reaction of adenine and adenosine at the copp er electrode could be observed in the solution, if PO43- and citric ac id exist simultaneously. The oxidation reaction of adenine and adenosi ne could be applied to capillary zone electrophoresis with end-column amperometric detection for their determination. The method was applied to the determination of adenine in yeast-RNA.