SIMULTANEOUS DETERMINATION OF CEPHALEXIN AND CARBOCISTEINE FROM CAPSULES BY REVERSE-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY (RP-HPLC)

A new, simple, precise,accurate and rapid RP- HPLC method has been dev eloped for the simultaneous determination of Cephalexin and Carbociste ine from capsules, using 0.025 M monobasic sodium phosphate : acetonit rile (87 : 13, v/v) as a mobile phase, and a C-8 Shodex column as the stationary phase. Detection was carried out using a UV detector at 210 nm. The linearity range for Cephalexin and Carbocisteine were 50 - 30 0 mu g/mL and 40 - 200 mu g/mL, respectively. Assay values for Cephale xin and Carbocisteine were 99.46% (R.S.D. - 1.30%) and 99.22% (R.S.D. - 1.34%) for Brand 1, and 99.71% (R.S.D. - 1.68%) and 99.43% (R.S.D. - 1.78%) for Brand 2, respectively.