Difficulties overwintering container stock in northern Ontario led to
the development of the ''extended greenhouse culture'' hardening regim
e for spruce seedlings. Laboratories to measure shoot frost hardiness
and evaluate terminal bud development were established to monitor nurs
ery crops being hardened using this regime. information on frost hardi
ness and bud development provided by these laboratories has been used
by nursery managers to determine readiness of container seedlings for
overwintering. Since 1982, over 200 stock lots have been monitored by
these operational laboratories. This database can be used to determine
the importance of nursery cultural factors and seed source on frost h
ardening. The database shows large differences between nurseries in ap
proach to hardening seedlings which were reflected in levels of freezi
ng damage, winter desiccation and overwintering success. Rates of fros
t hardening (i.e., the interval between terminal bud initiation and at
tainment of a -15 degrees C level of shoot frost hardiness) of crops p
roduced in north central Ontario failed to show significant seed sourc
e effects. The rate of frost hardening was faster in crops producing f
ewer needle primordia in terminal buds.