ENZYMATIC METHOD FOR THE SPECTROPHOTOMETRIC DETERMINATION OF BENZOIC-ACID IN SOY-SAUCE AND PICKLES

Authors
HAMANO T MITSUHASHI Y AOKI N SEMMA M ITO Y
Citation
T. Hamano et al., ENZYMATIC METHOD FOR THE SPECTROPHOTOMETRIC DETERMINATION OF BENZOIC-ACID IN SOY-SAUCE AND PICKLES, Analyst, 122(3), 1997, pp. 259-262
Citations number
11
Categorie Soggetti
Chemistry Analytical
Journal title
Analyst → ACNP
ISSN journal
00032654
Volume
122
Issue
3
Year of publication
1997
Pages
259 - 262
Database
ISI
SICI code
0003-2654(1997)122:3<259:EMFTSD>2.0.ZU;2-R
Abstract
A simple spectrophotometric method for the determination of benzoic ac id is described. Benzoic acid is measured enzymically through its reac tion with benzoate 4-hydroxylase coupled with NADPH and O-2. The entir e enzymic procedure required 20 min to complete. The method greatly si mplifies the procedure for benzoic acid determination and permits the routine inspection of a number of samples with very little laboratory equipment. The method was compared with HPLC and satisfactory agreemen t was achieved.