IMMOBILIZATION OF ORGANIC SOLVENT-SOLUBLE LIPASE IN NONAQUEOUS CONDITIONS AND PROPERTIES OF THE IMMOBILIZED ENZYMES

Lipid-coated Pseudomonas lipase was successfully immobilized by entrap ping with hydrophobic, photo-crosslinkable, resin prepolymers under no n-aqueous conditions. We studied the feasibility of using this new typ e of biocatalyst, which is an immobilized organic solvent-soluble enzy me, for enzymatic synthesis in organic solvent. Esterification of laur ic acid with benzyl alcohol in benzene and transesterification between racemic sulcatol and isopropenyl acetate in different organic solvent s were carried out with the immobilized lipid-coated lipase as the mod el reactions. In both reaction systems, the immobilized organic solven t-soluble enzyme was observed to have excellent activity and stability . (C) 1996 Elsevier Science B.V. All rights reserved.