Xh. Ma et Yl. Shi, EFFECTS OF ADP, DTT, AND MG2-CONDUCTIVE PROPERTY OF CHLOROPLAST H+-ATPASE(CF0-CF1) RECONSTITUTED INTO BILAYER-MEMBRANE( ON THE ION), Biochemical and biophysical research communications, 232(2), 1997, pp. 461-463
The purified CF0-CF1 complex of spinach was incorporated into planar l
ipid bilayer membranes (LBMs) formed with soybean phospholipid, and th
e transmembrane ion-transmission properties were studied under voltage
-clamp mode. The results showed that the presence of both ADP and Pi d
ecreased the membrane current while Dithiothreitol could evoke a stron
ger conductive change of CF0-CF1 containing LBMs when Ca2+ or Mg2+ exi
sts. Mg2+ can dramatically increase the CF0-CF1 conductance in various
conditions. These results indicated that the H+-transitive function o
f CF0-CF1 reconstituted in bilayer is sensitive to those factors which
can affect its ATP synthase activity in vivo. (C) 1997 Academic Press
.