EFFECTS OF ADP, DTT, AND MG2-CONDUCTIVE PROPERTY OF CHLOROPLAST H+-ATPASE(CF0-CF1) RECONSTITUTED INTO BILAYER-MEMBRANE( ON THE ION)

The purified CF0-CF1 complex of spinach was incorporated into planar l ipid bilayer membranes (LBMs) formed with soybean phospholipid, and th e transmembrane ion-transmission properties were studied under voltage -clamp mode. The results showed that the presence of both ADP and Pi d ecreased the membrane current while Dithiothreitol could evoke a stron ger conductive change of CF0-CF1 containing LBMs when Ca2+ or Mg2+ exi sts. Mg2+ can dramatically increase the CF0-CF1 conductance in various conditions. These results indicated that the H+-transitive function o f CF0-CF1 reconstituted in bilayer is sensitive to those factors which can affect its ATP synthase activity in vivo. (C) 1997 Academic Press .