SINGLE-PARTICLE TRACKING - THE DISTRIBUTION OF DIFFUSION-COEFFICIENTS

Authors
Citation
Mj. Saxton, SINGLE-PARTICLE TRACKING - THE DISTRIBUTION OF DIFFUSION-COEFFICIENTS, Biophysical journal, 72(4), 1997, pp. 1744-1753
Citations number
46
Categorie Soggetti
Biophysics
Journal title
ISSN journal
00063495
Volume
72
Issue
4
Year of publication
1997
Pages
1744 - 1753
Database
ISI
SICI code
0006-3495(1997)72:4<1744:ST-TDO>2.0.ZU;2-5
Abstract
In single-particle tracking experiments, the diffusion coefficient D m ay be measured from the trajectory of an individual particle in the ce ll membrane. The statistical distribution of single-trajectory diffusi on coefficients is examined by Monte Carlo calculations. The width of this distribution may be useful as a measure of the heterogeneity of t he membrane and as a test of models of hindered diffusion in the membr ane. For some models, the distribution of the short-range diffusion co efficient is much narrower than the observed distribution for proteins diffusing in cell membranes. To aid in the analysis of single-particl e tracking measurements, the distribution of D is examined for various definitions of D and for various trajectory lengths.