REFOLDING OF SOLUBLE LEUKEMIA INHIBITORY FACTOR-RECEPTOR FUSION PROTEIN (GP-190 SOL DAF) FROM UREA

Authors
LIU HQ MOREAU JF GUALDE N FU JL
Citation
Hq. Liu et al., REFOLDING OF SOLUBLE LEUKEMIA INHIBITORY FACTOR-RECEPTOR FUSION PROTEIN (GP-190 SOL DAF) FROM UREA, Molecular and cellular biochemistry, 169(1-2), 1997, pp. 43-50
Citations number
16
Categorie Soggetti
Biology,"Cell Biology
Journal title
Molecular and cellular biochemistry → ACNP
ISSN journal
03008177
Volume
169
Issue
1-2
Year of publication
1997
Pages
43 - 50
Database
ISI
SICI code
0300-8177(1997)169:1-2<43:ROSLIF>2.0.ZU;2-#
Abstract
The insoluble inclusion bodies of soluble leukemia inhibitory factor r eceptor fusion protein (gp 190 sol DAF) was solubilized in 8 M urea on the unfolding transitions, and several factors on the aggregate forma tion were indirectly analyzed for the refolding of EP 190 sol DAF. Res ults indicate that the refolding yield can be considerably increased a t lowering concentration of the unfolding protein, a little soluble pr otein with the slow refolding appears in the process of the aggregate formation and the concentration of the denaturant must be down to a mi nimum level for its refolding.