Rg. Shah et al., DETERMINATION OF GENOTOXICITY OF THE METABOLITES OF THE PESTICIDES GUTHION, SENCOR, LOROX, REGLONE, DACONIL AND ADMIRE BY P-32 POSTLABELING, Molecular and cellular biochemistry, 169(1-2), 1997, pp. 177-184
Commercial formulations of the pesticides: Guthion (azinphos methyl),
Sencor (metribuzin), Lorox (linuron), Reglone (diquat), Daconil (chlor
othalonil) and Admire (imidacloprid) were studied for their genotoxici
ty by P-32-postlabeling. Metabolites of the pesticides were obtained e
nzymatically using arochlor induced rat liver S9 fraction, in an NADPH
generating system, The resulting metabolites were reacted with calf t
hymus DNA and the DNA was analyzed for presence of adducts by either t
he nuclease P1 or butanol enrichment. Nuclease P1 enrichment resulted
in adducts for all the pesticides. Compared to the level of adducts in
control DNA, the levels in pesticide-treated DNA were higher for all
the pesticides, except Daconil. The increase in adduct numbers for pes
ticide-treated DNAs ranged from 4.9-12.4 times the control-DNA indicat
ing pesticide genotoxicity in this in vitro system. Enrichment using b
utanol extraction gave three adducts unique to Sencor-DNA. These adduc
ts were different from those obtained with nuclease P1 enrichment of t
he same. B(alpha)P was the positive control for the in vitro metabolis
m, and two adduct enrichment procedures: nuclease P1 digestion and but
anol extraction.