F. Alfani et al., ENZYMATIC CONVERSION OF CEPHALOSPORIN-C INTO GLUTARYL 7-AMINOCEPHALOSPORANIC ACID - A STUDY IN DIFFERENT REACTOR CONFIGURATIONS, Biotechnology letters, 19(2), 1997, pp. 175-178
Conversion of cephalosporin C into glutaryl 7-aminocephalosporanic aci
d was catalysed by D-aminoacid oxidase from Trigonopsis variabilis, co
valently immobilized on the polystyrenic resin Duolite A365. Spontaneo
us degradation of substrates was limited without depressing enzymatic
activity at the optimum reaction pH 8.0. The highest product yield was
1.77 mmol per g of biocatalyst, attained at 15 degrees C in both batc
h stirred and fluidized-bed reactors.