ENZYMATIC CONVERSION OF CEPHALOSPORIN-C INTO GLUTARYL 7-AMINOCEPHALOSPORANIC ACID - A STUDY IN DIFFERENT REACTOR CONFIGURATIONS

Authors
ALFANI F CANTARELLA M CUTARELLA N GALLIFUOCO A GOLINI P BIANCHI D
Citation
F. Alfani et al., ENZYMATIC CONVERSION OF CEPHALOSPORIN-C INTO GLUTARYL 7-AMINOCEPHALOSPORANIC ACID - A STUDY IN DIFFERENT REACTOR CONFIGURATIONS, Biotechnology letters, 19(2), 1997, pp. 175-178
Citations number
6
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
19
Issue
2
Year of publication
1997
Pages
175 - 178
Database
ISI
SICI code
0141-5492(1997)19:2<175:ECOCIG>2.0.ZU;2-S
Abstract
Conversion of cephalosporin C into glutaryl 7-aminocephalosporanic aci d was catalysed by D-aminoacid oxidase from Trigonopsis variabilis, co valently immobilized on the polystyrenic resin Duolite A365. Spontaneo us degradation of substrates was limited without depressing enzymatic activity at the optimum reaction pH 8.0. The highest product yield was 1.77 mmol per g of biocatalyst, attained at 15 degrees C in both batc h stirred and fluidized-bed reactors.