Nd. Huebert et al., ANALYSIS OF 2-DIFLUOROMETHYL-DL-ORNITHINE IN HUMAN PLASMA, CEREBROSPINAL-FLUID AND URINE BY CATION-EXCHANGE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography, 762(1-2), 1997, pp. 293-298
Citations number
3
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
An analytical method has been developed based on cation-exchange liqui
d chromatography for the measurement of 2-difluoromethyl-DL-ornithine
(DFMO) in human plasma, cerebrospinal fluid (CSF) and urine. Fluoresce
nce detection at excitation-emission wavelengths of 340/440 nm is foll
owed by postcolumn derivatization with o-phthalaldehyde-2-mercaptoetha
nol. All calibration ranges yielded linear relationships with correlat
ion coefficients better than 0.999. In each case the limit of quantita
tion was equal to the lowest value of the standard curve. The variabil
ity of the assay, expressed as relative standard deviations, was less
than 7.1%, 15.3% and 7.1% for plasma, CSF and urine, respectively. The
accuracy of the assay (expressed as relative errors) ranged between -
4.3% and 2.0% for plasma analysis, between -0.1% and 14.0% for CSF ana
lysis and between -8.0% and 2.0% for urine analysis. Plasma, CSF and u
rinary DFMO concentrations were measured in samples obtained from pati
ents undergoing treatment for trypanosomiasis. The method was found to
be applicable for the measurement of DFMO levels in human body fluids
for the determination of pharmacokinetic parameters in clinical studi
es.