COMPLEX POLYSACCHARIDES AS PCR INHIBITORS IN FECES - HELICOBACTER-PYLORI MODEL

Authors
MONTEIRO L BONNEMAISON D VEKRIS A PETRY KG BONNET J VIDAL R CABRITA J MEGRAUD F
Citation
L. Monteiro et al., COMPLEX POLYSACCHARIDES AS PCR INHIBITORS IN FECES - HELICOBACTER-PYLORI MODEL, Journal of clinical microbiology, 35(4), 1997, pp. 995-998
Citations number
25
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
35
Issue
4
Year of publication
1997
Pages
995 - 998
Database
ISI
SICI code
0095-1137(1997)35:4<995:CPAPII>2.0.ZU;2-U
Abstract
A model was developed to study inhibitors present in feces which preve nt the use of PCR for the detection of Helicobacter pylori. A DNA frag ment amplified with the same primers as H. pylori was used to spike sa mples before extraction by a modified QIAamp tissue method. Inhibitors , separated on an Ultrogel AcA44 column, were characterized. Inhibitor s in feces are complex polysaccharides possibly originating from veget able material in the diet.