M. Joaquin et al., EXPRESSION OF THE F-TYPE 6-PHOSPHOFRUCTO-2-KINASE FRUCTOSE-2,6-BISPHOSPHATASE MESSENGER-RNA DURING LIVER-REGENERATION/, Biochimica et biophysica acta (G). General subjects, 1334(2-3), 1997, pp. 256-260
The expression of the F-type -phosphofructo-2-kinase/fructose-2,6-biph
osphatase mRNA was studied during liver regeneration by three independ
ent assays: Northern blot analysis, reverse transcription-polymerase c
hain reaction and ribonuclease protection. We demonstrate the presence
of F-type mRNA in foetal and adult rat livers and a transient increas
e in its levels with a maximum at 12 h after partial liver resection.
The time course of F-type mRNA induction differs from that reported fo
r the L-type isoform, suggesting differences in the regulation of the
expression of F- and L-type isoforms of the bifunctional enzyme during
liver regeneration.