EXPRESSION OF THE F-TYPE 6-PHOSPHOFRUCTO-2-KINASE FRUCTOSE-2,6-BISPHOSPHATASE MESSENGER-RNA DURING LIVER-REGENERATION/

The expression of the F-type -phosphofructo-2-kinase/fructose-2,6-biph osphatase mRNA was studied during liver regeneration by three independ ent assays: Northern blot analysis, reverse transcription-polymerase c hain reaction and ribonuclease protection. We demonstrate the presence of F-type mRNA in foetal and adult rat livers and a transient increas e in its levels with a maximum at 12 h after partial liver resection. The time course of F-type mRNA induction differs from that reported fo r the L-type isoform, suggesting differences in the regulation of the expression of F- and L-type isoforms of the bifunctional enzyme during liver regeneration.