PROBING OF PSEUDOMONAS-AERUGINOSA, PSEUDOMONAS-AUREOFACIENS, BURKHOLDERIA (PSEUDOMONAS)-CEPACIA, PSEUDOMONAS-FLUORESCENS, AND PSEUDOMONAS-PUTIDA WITH THE FERRIPYOCHELIN RECEPTOR-A GENE AND THE SYNTHESIS OF PYOCHELIN IN PSEUDOMONAS-AUREOFACIENS, PSEUDOMONAS-FLUORESCENS, AND PSEUDOMONAS-PUTIDA
D. Castignetti, PROBING OF PSEUDOMONAS-AERUGINOSA, PSEUDOMONAS-AUREOFACIENS, BURKHOLDERIA (PSEUDOMONAS)-CEPACIA, PSEUDOMONAS-FLUORESCENS, AND PSEUDOMONAS-PUTIDA WITH THE FERRIPYOCHELIN RECEPTOR-A GENE AND THE SYNTHESIS OF PYOCHELIN IN PSEUDOMONAS-AUREOFACIENS, PSEUDOMONAS-FLUORESCENS, AND PSEUDOMONAS-PUTIDA, Current microbiology, 34(4), 1997, pp. 250-257
The ferripyochelin receptor A (fptA) gene codes for the transport of t
he ferrisiderophore ferripyochelin in Pseudomonas aeruginosa. A P. aer
uginosa fptA internal fragment was used to probe chromosomal DNA from
P. aureofaciens, B. cepacia, P. fluorescens, P. putida, and five strai
ns of P. aeruginosa. These bacteria all contained DNA that hybridized
to the fptA fragment. Four of the five P. aeruginosa strains displayed
marked and identical patterns, indicating a high degree of sequence s
imilarities among these strains. DNA from the non-P. aeruginosa bacter
ia, in contrast, hybridized less to the fptA fragment. Pseudomonas aer
uginosa and B. cepacia synthesize pyochelin. Experiments were performe
d to confirm P. fluorescens pyochelin synthesis and to determine if py
ochelin, cepabactin or salicylic acid were made by P. aureofaciens, P.
putida, and P. fluorescens. Only pyochelin was isolated and identifie
d from P. fluorescens. P. aureofaciens and P. putida produced none of
these compounds. While all of these bacteria contain chromosomal DNA t
hat hybridized to the fptA fragment probe, pyochelin synthesis did not
occur in all, indicating that fptA fragment hybridization cannot alwa
ys be correlated with pyochelin biosynthesis.