GIGANTOCELLULAR VASODEPRESSOR AREA IS TONICALLY ACTIVE AND DISTINCT FROM CAUDAL VENTROLATERAL VASODEPRESSOR AREA

The gigantocellular depressor area (GiDA) is a functionally defined su bdivision of the medullary gigantocellular reticular formation where v asodepressor responses are evoked by glutamate microinjections (Aicher , S. A., D. J. Reis, D. A. Ruggiero, and T. A. Milner. Neuroscience 60 : 761-779, 1994). The present experiments sought to determine whether the GiDA 1) tonically inhibits the sympathetic nervous system; 2) is n ecessary for baroreflex function; and 3) is functionally distinct from adjacent vasodepressor regions in the medullary reticular formation, including the midline raphe nuclei and the caudal ventrolateral medull a (CVL). Excitotoxic lesions of the GiDA abolished the baroreflex and significantly increased sympathetic nerve activity in anesthetized rat s. Equivalent injections into the midline raphe nuclei elevated sympat hetic activity but did not alter baroreflex responses. Therefore, the GiDA is functionally distinct from the raphe nuclei, although both con tain tonically active sympathoinhibitory neurons. Because the effects of GiDA lesions were identical to those seen after lesions of the CVL, further studies were required to demonstrate that the GiDA and CVL ar e functionally and anatomically distinct. First, intramedullary inject ions of kynurenic acid produced hypertension and blocked the barorefle x when placed in the CVL, but not when placed in the GiDA. Second, mus cimol inactivation of the RVL blocked the hypertension produced by exc itotoxic lesions of the CVL, but failed to block the hypertension prod uced by similar lesions of the GiDA. Third, CVL neurons project to the RVL but not the spinal cord, whereas GiDA neurons project to the spin al cord but not the RVL. These studies show that the CVL and GiDA are both tonically sympathoinhibitory regions, but they are distinct with regard to their functional connectivity with other autonomic regions.