BLOOD COUNTS IN HEALTHY DONORS 1 YEAR AFTER THE COLLECTION OF GRANULOCYTE-COLONY-STIMULATING FACTOR-MOBILIZED PROGENITOR CELLS AND THE RESULTS OF A 2ND MOBILIZATION AND COLLECTION

BACKGROUND: Granulocyte-colony-stimulating factor (G-CSF)-mobilized bl ood cells are being used for allogeneic transplants. but the long-term effects of G-CSF on healthy individuals are not known. Furthermore, i t is not certain how many CD34+ cells can be collected in a second mob ilization and collection procedure. STUDY DESIGN AND METHODS: Nineteen people were given 2, 5,7.5, or 10 mu g of G-CSF per kg per day for 5 days, and blood progenitor cells were collected by apheresis on the si xth day. this was done on two occasions separated by at least 12 month s. Blood counts obtained before and after each course of G-CSF and the quantity of cells collected were compared. RESULTS: There were no dif ferences in white cell (WBC), platelet, red cell, and WBC differential counts measured before each course of G-CSF, and all the values were in the normal range. In a subset of 12 people who received 7.5 or 10 p g of G-CSF per leg per day for both courses; the numbers of neutrophil s, mononuclear cells, and CD34+ cells in the blood after each course w ere similar (34.1 +/- 7.31 x 10(9)/L vs. 36.4 +/- 12.3 x 10(9)/L, p = 0.24; 6.59 +/- 2.28 x 10(9)/L vs. 5.63 +/- 2.11 x 10(9)/L, p = 0.24; a nd 92.0 +/- 55.6 x 10(6)/L vs. 119.2 +/- 104.6 x 10(6)/L; p = 0.48, re spectively), as were the quantities of mononuclear cells (31.0 +/- 8.4 x 10(9) vs. 31.0 +/- 6.1 x 10(9); p = 0.64) and CD34+ cells (417 +/- 353 x 10(6) VS. 449 +/- 286 x 10(6); p = 0.53) collected in the two ap heresis procedures. Furthermore, there was a positive correlation betw een the quantity of CD34+ cells collected from each of the 12 people p er liter of whole blood processed in the two procedures(r(2) = 0.86, p <0.001).CONCLUSION: One year alter the administration of G-CSF to heal thy people, their blood counts were normal and unchanged from pretreat ment counts. if healthy people donate blood progenitor cells after a s econd G-CSF course, the quantity of CD34+ cells collected will be simi lar to that obtained in the first collection.