M. Kobayashi et al., 2-DIMENSIONAL IMAGING OF ULTRAWEAK PHOTON-EMISSION FROM GERMINATING SOYBEAN SEEDLINGS WITH A HIGHLY SENSITIVE CCD CAMERA, Photochemistry and photobiology, 65(3), 1997, pp. 535-537
A novel application of a highly sensitive charge-coupled device (CCD)
camera for imaging of spontaneous ultraweak photon emission from livin
g organisms (biophoton emission) is described, The performance of the
CCD camera for low-level light imaging is theoretically compared to a
conventionally used two-dimensional photon counting tube, and we deduc
e that in the wavelength region above 700 nm and for measurement perio
ds over 2000 s, the CCD camera is advantageous over the two-dimensiona
l photon counting tube, Experimental results on the two-dimensional bi
ophoton imagery of germinating soybean seedlings also suggest that the
wide-range spectral sensitivity of the CCD camera is effective for lo
w-level light imaging from living organisms that have a predominant em
ission spectrum in the red and near-IR wavelength region.