Dl. Bloxam et al., CULTURE OF SYNCYTIOTROPHOBLAST FOR THE STUDY OF HUMAN PLACENTAL-TRANSFER .2. PRODUCTION, CULTURE AND USE OF SYNCYTIOTROPHOBLAST, Placenta, 18(2-3), 1997, pp. 99-108
The conditions necessary for producing syncytical syncytiotrophoblast
are examined. Tissue disaggregation conditions, culture media composit
ion, different extracellular matrices and the influence of placental g
estational age are all assessed. The importance of evaluating the bioc
hemical and functional differentiational state of the cells is also st
ressed. Evidence is summarized that syncytiotrophoblast in culture is
morphologically and ultrastructurally very similar to syncytiotrophobl
ast in vivo, and what is so far known biochemically is largely consist
ent with what is known in vivo. Studies published to date on microvill
ous membrane uptake and release and relationships with intracellular m
etabolism using syncytiotrophoblast in conventional culture are outlin
ed from the point of view of the advantages and potential of this mode
l. The present state of development of the two-sided model is assessed
, mentioning factors to be considered such as the supporting membrane
to be used, accounting for passive diffusion and paracellular leak com
ponents of transport and dealing with quantitative effects in kinetic
studies of the presence of the supporting membrane. It is concluded th
at satisfactory methods are now in place for preparing pure villous sy
ncytial syncytiotrophoblast in culture from cytotrophoblast derived fr
om term (but not early) placentae, suitable for studying microvillous
membrane transport and relationships with intracellular metabolism. Cy
totrophoblast from early gestational age placenta may require differen
t conditions to form true syncytiotrophoblast. A two-sided model for s
tudies of overall transfer, basal transport and basal control mechanis
ms is now available and possibly with some development should be a goo
d model for such investigations. (C) 1997 W. B. Saunders Company Ltd.